HAKKIMIZDA

11th HANDS-ON WORKSHOP ON PGD FOR GENETIC AND CHROMOSOMAL DISORDERS BY TROPHECTODERM BIOPSY

Anasayfa / Hakkımızda / Bilimsel Toplantılar

11th HANDS-ON WORKSHOP ON PGD FOR GENETIC AND CHROMOSOMAL DISORDERS BY TROPHECTODERM BIOPSY PGDIS Workshop: APRIL 29 – MAY 01, 2018 ISTANBUL, TURKEY






GDIS HANDS-ON WORKSHOP ON PGT FOR GENETIC AND CHROMOSOMAL DISORDERS BY TROPHECTODERM BIOPSY

Memorial Şişli Hospital ART and Genetics Center, Istanbul

 

April 29-May 1, 2018

 

April 28 Arrival & Accommodation

 

APRIL 29

 

09:00-10:05

Preimplantation Genetic Testing (PGT) for Monogenic and

Chromosomal Disorders by Blastomere and Trophectoderm Biopsy - Theoretical

 

 

Session 1

 

09:00 - 09:05

Opening of Workshop, Semra KAHRAMAN, Memorial Hospital ART and Genetics Center, Istanbul, Turkey

08:30 - 09:00

Registration

 

09:05 - 09:35

Practical PGT, Anver KULIEV, RGI, Chicago, USA

 

09:35 -10:05

Advantages and disadvantages of trophectoderm biopsy – Caroline Selma PIRKEVI CETINKAYA, Memorial Hospital ART and Genetics Center, Istanbul, Turkey

10:05 -10.35

DISCUSSION & COFFEE BREAK

10:35 -13:10

Hands-on Applications of Biopsy Procedures under Supervision of Trainers

Participants will be divided into 3 groups, up to 5 in each for practical sessions.

Zona Drilling Methods

Blastomere Biopsy Techniques

Trophectoderm Biopsy Techniques

13:10 -14:20

LUNCH & DISCUSSION

14:20 -18:30

Each group will apply all the demonstrated techniques

   

APRIL 30

 

09:00 - 10:00

PGT for Monogenic and Chromosomal Disorders by Blastomere and Trophectoderm Biopsy- Theoretical Session 2

09:00 - 09:20

Clinical aspects of successful PGT, Semra KAHRAMAN, Memorial Hospital ART and Genetics Center, Istanbul, Turkey

09:20 - 09:40

PGT for Aneuploidy (PGT-A) and Structural Rearrangements (PGT-SR)- From FISH to Next Generation Technologies, Anver KULIEV, RGI, Chicago, USA

09:40 - 10:00

Molecular Techniques and Next Generation Technologies in PGT: Testing for Monogenic Disorders (PGT-M), HLA Typing (PGT-HLA), and 24 Chromosome Aneuploidy (PGT-A) by Next Generation Sequencing (NGS), Don LEIGH, Sydney, Australia

10:00 -10:30

DISCUSSION & COFFEE BREAK

10:30 -13:30

Hands-on applications of trophectoderm biopsy by participants under supervision of trainers

13:30 -14:30

LUNCH & DISCUSSION

14:30 -18:30

Hands-on Applications of FISH and PCR techniques under supervision of trainers

Preparation, Spreading and Fixation of Blastomere and Trophectoderm Cells

In Situ Hybridization with Different Chromosome Specific Probes

Analysis and Scoring of FISH Signals

1st and 2nd Round PCR for PGT-M and PGT- HLA

Testing for 24 Chromosomes by Microarray Technology and NGS

Participants will be divided into 3 groups, up to 5 in each for practical sessions according to their interests.

   

May 1

 

09:00 -13:00

Interpreting NGS, aCGH, FISH and PCR Results

09:00 - 12:00

Analysis and Interpreting NGS, arrayCGH and FISH results, Murat CETINKAYA, Memorial Hospital ART and Genetics Center, Istanbul, Turkey

Analysis and Interpretation of NGS and PCR Results, Don LEIGH, Syndey, Australia

12:00 -13:00

GENERAL DISCUSSION & CLOSING

Practical Stations

Station 1: Blastomere Biopsy Techniques;

Zona hatchings by enzymatic, mechanical and laser applications

Station 2: Trophectoderm Biopsy Techniques;

Station 3: FISH Techniques;

Preparation, spreading and fixation of Blastomere and Trophectoderm Cells In situ hybridization of chromosomes with different probes

Analysis and Scoring of FISH signals

Station 4: array CGH and NGS Techniques;

Extraction and Amplification of blastomere and trophectoderm cells Labeling Hybridization

Scanning and analysis of aCGH and NGS results

Station 5: PCR Techniques;

Lysis of blastomere and trophectoderm cells

First and second round PCR for PGT-M and PGT-HLA, Mutation Analysis and Restriction Enzyme Digestion Analysis and interpretation of PCR results


  • These applications
    will be done simultaneously in 4 stations and each participant will attend to each station and perform all demonstrated techniques

 

SAYFA BAŞINA DÖN