Istanbul Memorial Hospital ART and Genetics Center April 29-May 1, 2018
Chromosomal Disorders by Blastomere and Trophectoderm Biopsy - Theoretical
Session 1
08:30 - 09:00 Registration
09:00 - 09:05 Opening of Workshop, Semra KAHRAMAN, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
09:05 - 09:35 Practical PGT, Anver KULIEV, RGI, Chicago, USA
09:35 -10:05 Advantages and disadvantages of trophectoderm biopsy – Caroline Selma PIRKEVI CETINKAYA, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
10:05 -10.35 DISCUSSION & COFFEE BREAK
Participants will be divided into 3 groups, up to 5 in each for practical sessions.
09:00 - 10:00 PGT for Monogenic and Chromosomal Disorders by Blastomere and Trophectoderm Biopsy- Theoretical Session 2
09:00 - 09:20 Clinical aspects of successful PGT, Semra KAHRAMAN, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
09:20 - 09:40 PGT for Aneuploidy (PGT-A) and Structural Rearrangements (PGT-SR)- From FISH to Next Generation Technologies, Anver KULIEV, RGI, Chicago, USA
09:40 - 10:00 Molecular Techniques and Next Generation Technologies in PGT: Testing for Monogenic Disorders (PGT-M), HLA Typing (PGT-HLA), and 24 Chromosome Aneuploidy (PGT-A) by Next Generation Sequencing (NGS), Don LEIGH, Sydney, Australia
10:00 -10:30 DISCUSSION & COFFEE BREAK
10:30 -13:30 Hands-on applications of trophectoderm biopsy by participants under supervision of trainers 13:30 -14:30 LUNCH & DISCUSSION
Participants will be divided into 3 groups, up to 5 in each for practical sessions according to their interests.
09:00 -13:00 Interpreting NGS, aCGH, FISH and PCR Results
09:00 - 12:00 Analysis and Interpreting NGS, arrayCGH and FISH results, Murat CETINKAYA, Memorial Hospital ART and Genetics Center, Istanbul, Turkey
Analysis and Interpretation of NGS and PCR Results, Don LEIGH, Syndey, Australia 12:00 -13:00 GENERAL DISCUSSION & CLOSING
Station 1: Blastomere Biopsy Techniques;
Zona hatchings by enzymatic, mechanical and laser applications
Station 2: Trophectoderm Biopsy Techniques;
Station 3: FISH Techniques;
Preparation, spreading and fixation of Blastomere and Trophectoderm Cells In situ hybridization of chromosomes with different probes
Analysis and Scoring of FISH signals
Station 4: array CGH and NGS Techniques;
Extraction and Amplification of blastomere and trophectoderm cells Labeling Hybridization
Scanning and analysis of aCGH and NGS results Station 5: PCR Techniques;
Lysis of blastomere and trophectoderm cells
First and second round PCR for PGT-M and PGT-HLA, Mutation Analysis and Restriction Enzyme Digestion Analysis and interpretation of PCR results